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HYBRID MAGNETIC CONSTRUCTIONS AS A BASIS FOR CREATION OF EFFECTIVE MAGNETO-SENSITIVE BIOSENSORS

E-mail
31.05.2011 .
Siberian State Medical University, Researcher of Central Research Laboratory, Russia

XII         " "  (, 26-27 2011 .) / .   .. , .. . – : . – 2011. – 104 . 

(PDF, 1,6 MB)

The development of hybrid magnetic nanobiostructures, which are able to bind to target biomolecule in a specific way, opens some attractive opportunities in the area of high-sensitivity diagnostic systems development [1].

The goal of this research was to examine the functional activity of the proteins immobilized on nano- particles surface. The carbon coated iron nanoparticles served as a core of the developed construction [2]. Bovine serum albumin, interferon alpha and horseradish peroxidase were used as model biomolecules. For covalent immobilization of proteins the surface of nanoparticles was modified with original diazonium salt - 4- carboxy benzenediazonium tosylate [3]. The proteins were covalently bound to nanoparticles by carbo- diimide activation. The nanohybryd constructions received as the result, were separated from the mixture by magnetic precipitation and centrifugation and washed with phosphate buffer and water at least four times.

The protein-nanoparticles complex formation was confirmed by FT-IR spectroscopy (in IR-spectra of complexes characteristic absorption bands of protein appeared in the region of 1600-1500 cm-1). Spectrophotometry analysis has shown that protein -binding capacity of nanoparticles constituted 0,1-0,4*10-6 Mol per gramm. It was determined, that horseradish peroxidase partially retained the specific enzymatic activity after immobilization. The capability of nanoparticles containing interferon alpha to bind with the specific antibodies was demonstrated by ELISA. Therefore the immobilization doesn't lead to the distortion of the protein molecule spatial structure.

There was developed the fundamental approach to the creation of the hybrid bionanocomposite constructions which include the magnetic nanoparticles covalently bound to proteins. This approach constitutes the main prerequisite for the development of new generation effective magneto-sensitive biosensors.

Reference:

  1. Tan W. et al. // Med. Res. Rev. - 2004. - V. 5. - . 621
  2. Ermakov A.E. et al. // . . - 2009. - V. 83(7). - . 1338-1345
  3. . . // . . - 2010. - . 5(7-8). - . 49-51
 

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